6QSA
Cryogenic temperature structure of blue light-irradiated AtPhot2LOV2 recorded after an accumulated dose of 48 kGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 40.324, 40.324, 131.281 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.550 - 1.700 |
R-factor | 0.19088 |
Rwork | 0.190 |
R-free | 0.21629 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eep |
RMSD bond length | 0.005 |
RMSD bond angle | 1.642 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.550 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 12659 | 1199 |
<I/σ(I)> | 10.39 | 1.73 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 6.24 | 6.45 |
CC(1/2) | 0.998 | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 12-17% PEG8000 0.2 M Calcium Acetate 0.1 M MES |