6QQH
Cryogenic temperature structure of the ground state of AtPhot2LOV2 recorded after an accumulated dose of 2.68 MGy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-23 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 40.148, 40.148, 131.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.400 - 1.380 |
| R-factor | 0.14121 |
| Rwork | 0.140 |
| R-free | 0.17034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eep |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.354 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.400 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Number of reflections | 23065 | 2265 |
| <I/σ(I)> | 16.84 | 1.68 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.18 | 6.32 |
| CC(1/2) | 0.999 | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 12-17 % PEG8000 0.2 M Calcium acetate 0.1 M MES |
