6QQH

Cryogenic temperature structure of the ground state of AtPhot2LOV2 recorded after an accumulated dose of 2.68 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-23
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.968
Spacegroup name	P 43 21 2
Unit cell lengths	40.148, 40.148, 131.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.400 - 1.380
R-factor	0.14121
Rwork	0.140
R-free	0.17034
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eep
RMSD bond length	0.006
RMSD bond angle	1.354
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.400	1.430
High resolution limit [Å]	1.380	1.380
Number of reflections	23065	2265
<I/σ(I)>	16.84	1.68
Completeness [%]	99.8	99.8
Redundancy	7.18	6.32
CC(1/2)	0.999	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	12-17 % PEG8000 0.2 M Calcium acetate 0.1 M MES