6QQF
Room temperature structure of Hen Egg White Lysozyme recorded after an accumulated dose of 100 kGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2018-08-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.07 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.010, 79.010, 38.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.510 - 1.950 |
R-factor | 0.15675 |
Rwork | 0.152 |
R-free | 0.22987 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.426 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.510 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 9218 | 665 |
<I/σ(I)> | 10.45 | 2.73 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 6.7 | 7.2 |
CC(1/2) | 0.997 | 0.708 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 6-9% NaCl, 0.1M Na Acetate pH4.8 |