6QQE
Room temperature structure of Hen Egg White Lysozyme recorded after an accumulated dose of 20 kGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.07 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.149, 79.149, 38.097 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.610 - 1.370 |
R-factor | 0.15985 |
Rwork | 0.158 |
R-free | 0.18750 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.342 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.620 | 1.410 |
High resolution limit [Å] | 1.370 | 1.370 |
Number of reflections | 25762 | 1828 |
<I/σ(I)> | 17.1 | 2.24 |
Completeness [%] | 98.9 | 96.9 |
Redundancy | 7 | 7 |
CC(1/2) | 0.999 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 6-9% NaCl, 0.1M Na Acetate pH 4.8 |