6QQD
Cryogenic temperature structure of Hen Egg White Lysozyme recorded after an accumulated dose of 10 MGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.960, 77.960, 37.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.710 - 1.920 |
R-factor | 0.15577 |
Rwork | 0.152 |
R-free | 0.21594 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.572 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.710 | 1.970 |
High resolution limit [Å] | 1.920 | 1.920 |
Number of reflections | 8490 | 640 |
<I/σ(I)> | 15.34 | 1.88 |
Completeness [%] | 91.8 | 95.7 |
Redundancy | 6.4 | 6.6 |
CC(1/2) | 0.999 | 0.706 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 6-9% NaCl, 0.1M Na Acetate pH 4.8 |