6QQC
Cryogenic temperature structure of Hen Egg White Lysozyme recorded after an accumulated dose of 110 kGy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.560, 77.560, 37.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.490 - 1.420 |
| R-factor | 0.16924 |
| Rwork | 0.167 |
| R-free | 0.20351 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.780 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.490 | 1.460 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Number of reflections | 20446 | 1472 |
| <I/σ(I)> | 17.49 | 1.99 |
| Completeness [%] | 93.1 | 92.5 |
| Redundancy | 6.5 | 6.8 |
| CC(1/2) | 1.000 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 6-9% NaCl et 0.1M Na Acetate pH 4.8. |
