6QQB
Room temperature structure of the fluorescent protein Cerulean recorded after an accumulated dose of 147 kGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2014-12-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.040, 62.670, 71.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.150 - 2.450 |
R-factor | 0.18192 |
Rwork | 0.177 |
R-free | 0.26592 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.570 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.150 | 2.510 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 9048 | 670 |
<I/σ(I)> | 8.9 | 2.08 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.6 | 7.4 |
CC(1/2) | 0.997 | 0.749 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% PEG 8000, 0.1M MGCL2, 0.1 M HEPES |