6QQ9

Cryogenic temperature structure of the fluorescent protein Cerulean recorded after an accumulated dose of 5.8 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	51.410, 63.000, 70.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.990 - 1.820
R-factor	0.15432
Rwork	0.152
R-free	0.19519
RMSD bond length	0.009
RMSD bond angle	1.382
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.990	1.870
High resolution limit [Å]	1.820	1.820
Number of reflections	21133	1544
<I/σ(I)>	28.23	2.05
Completeness [%]	99.9	100
Redundancy	6.8	7.3
CC(1/2)	1.000	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100mM HEPES 7.0, 12% PEG8000, 100mM MgCl2