6QQ3
The room temperature structure of lysozyme via the acoustic levitation of a droplet
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 294 |
| Detector technology | PIXEL |
| Collection date | 2018-12-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.448, 79.448, 37.882 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.724 - 1.530 |
| R-factor | 0.1784 |
| Rwork | 0.177 |
| R-free | 0.20070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kxo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.800 |
| Data reduction software | DIALS |
| Data scaling software | cctbx.prime |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.740 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 18767 | 884 |
| <I/σ(I)> | 3.52 | 0.71 |
| Completeness [%] | 99.5 | 95 |
| Redundancy | 101.5 | 8.8 |
| CC(1/2) | 0.984 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 291 | 10 % w/v NaCl, 25 % w/v ethylene glycol, 100 mM Na Acetate pH 4.8 |
