6QPP
Rhizomucor miehei lipase propeptide complex, native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.040, 61.020, 62.650 |
| Unit cell angles | 90.00, 111.97, 90.00 |
Refinement procedure
| Resolution | 50.700 - 1.490 |
| R-factor | 0.13978 |
| Rwork | 0.138 |
| R-free | 0.17815 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tgl |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.850 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.700 | 1.530 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.064 | 0.270 |
| Rmeas | 0.085 | 0.364 |
| Number of reflections | 55513 | 368 |
| <I/σ(I)> | 9.8 | 3.8 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.996 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG8K, sodium cacodylate, ammonium sulphate |
