6QPH
Dunaliella minimal PSI complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-07-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 158.881, 100.506, 191.040 |
Unit cell angles | 90.00, 91.73, 90.00 |
Refinement procedure
Resolution | 48.947 - 3.400 |
R-factor | 0.3377 |
Rwork | 0.337 |
R-free | 0.35630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l8r |
RMSD bond length | 0.008 |
RMSD bond angle | 1.876 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.947 | 3.500 |
High resolution limit [Å] | 3.400 | 3.400 |
Rpim | 0.080 | 0.636 |
Number of reflections | 83250 | 4575 |
<I/σ(I)> | 8.2 | |
Completeness [%] | 99.9 | |
Redundancy | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | Sodium cacodylate 50mM, MgCl2 100mM, PEG1500 8-11% |