6QPF

Influenza A virus Polymerase Heterotrimer A/duck/Fujian/01/2002(H5N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	337.122, 192.859, 235.688
Unit cell angles	90.00, 91.46, 90.00

Refinement procedure

Resolution	235.611 - 3.634
R-factor	0.2794
Rwork	0.277
R-free	0.32290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d98
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	235.611	235.610	4.050
High resolution limit [Å]	3.634	12.160	3.850
Rmerge	0.484	0.254	2.539
Rmeas	0.576	0.302	3.013
Rpim	0.310	0.163	1.608
Number of reflections	143100	4659	20878
<I/σ(I)>	1.5
Completeness [%]	100.0	100	100
Redundancy	3.4	3.2	3.5
CC(1/2)	0.880	0.882	0.375

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	1M Phosphate Potassium Sodium pH6.9, 0.07% Dichloromethane