6QP8

Drosophila Semaphorin 2b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-04-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.27165
Spacegroup name	P 21 21 21
Unit cell lengths	107.990, 145.580, 152.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.667 - 2.330
R-factor	0.197
Rwork	0.195
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1olz
RMSD bond length	0.003
RMSD bond angle	0.554
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_3386: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.667	2.357
High resolution limit [Å]	2.330	2.330
Rmerge	0.182
Number of reflections	101324
<I/σ(I)>	10.45
Completeness [%]	98.4
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293.15	0.1 M trisodium citrate (pH 5.0), 10% PEG 6000