6QP7

Drosophila Semaphorin 2a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	103.804, 109.977, 134.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.464 - 1.960
R-factor	0.1847
Rwork	0.183
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1olz
RMSD bond length	0.003
RMSD bond angle	0.613
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_3386: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.464	1.982
High resolution limit [Å]	1.960	1.960
Rmerge	0.175
Number of reflections	110704
<I/σ(I)>	11.51
Completeness [%]	99.6
Redundancy	14.8
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	0.1 M Bis-Tris (pH 6.5) and 20% (w/v) PEG 5000 MME