6QNW

Influenza A Polymerase Heterotrimer Human H3N2 Northern Territory 1968

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.968620
Spacegroup name	C 1 2 1
Unit cell lengths	336.480, 191.895, 235.735
Unit cell angles	90.00, 91.50, 90.00

Refinement procedure

Resolution	138.620 - 3.310
R-factor	0.2377
Rwork	0.235
R-free	0.27870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d9a
RMSD bond length	0.002
RMSD bond angle	0.618
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	235.654	3.916
High resolution limit [Å]	3.310	3.321
Rmerge	0.459	2.243
Rmeas	0.476	2.363
Rpim	0.126	0.626
Number of reflections	123159	9466
<I/σ(I)>	4.8	2
Completeness [%]	95.3	80.7
Redundancy	14.4	13.7
CC(1/2)	0.988	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	1-1.6 M K/Na phosphate