6QNR

70S ribosome elongation complex (EC) with experimentally assigned potassium ions

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-11-10
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	209.460, 446.200, 623.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	223.100 - 3.100
R-factor	0.1981
Rwork	0.196
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v6f
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	223.100	6.000	3.200
High resolution limit [Å]	3.100	5.850	3.100
Rmerge			0.600
Number of reflections	1043872	22459	94202
<I/σ(I)>	6.94	1	1.9
Completeness [%]	96.5	99.6	88.4
Redundancy	9.1		8.86

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tris-OAc, KSCN, PEG-20k, PEG-550mme
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tris-OAc, KSCN, PEG-20k, PEG-550mme
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tris-OAc, KSCN, PEG-20k, PEG-550mme
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tris-OAc, KSCN, PEG-20k, PEG-550mme
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tris-OAc, KSCN, PEG-20k, PEG-550mme
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tris-OAc, KSCN, PEG-20k, PEG-550mme
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tris-OAc, KSCN, PEG-20k, PEG-550mme
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tris-OAc, KSCN, PEG-20k, PEG-550mme