6QNK

Antibody FAB fragment targeting Gi protein heterotrimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	169.940, 68.080, 133.670
Unit cell angles	90.00, 127.20, 90.00

Refinement procedure

Resolution	45.920 - 1.900
R-factor	0.1805
Rwork	0.179
R-free	0.21220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	12e8
RMSD bond length	0.005
RMSD bond angle	0.780
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX ((1.13_2998-000))
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.090	7.961
Rmeas	0.107	9.351
Rpim	0.057	4.863
Number of reflections	94811	9427
<I/σ(I)>	7.2	0.1
Completeness [%]	97.9	98.1
Redundancy	3.5	3.6
CC(1/2)	0.998	0.040

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.5 M (pH 7.5) malic acid, 7 % (v/v) lauryldimethylamine N-oxide.