6QN8

Structure of bovine anti-RSV Fab B13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 1 21 1
Unit cell lengths	87.908, 132.457, 118.183
Unit cell angles	90.00, 102.93, 90.00

Refinement procedure

Resolution	87.070 - 2.120
R-factor	0.23687
Rwork	0.235
R-free	0.26700
RMSD bond length	0.009
RMSD bond angle	1.449
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.800	2.160
High resolution limit [Å]	2.120	2.120
Rmerge	0.128
Number of reflections	148202	7273
<I/σ(I)>	19.4
Completeness [%]	99.2	97.6
Redundancy	6.8	6
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20.0% w/v Polyethylene glycol 3350 0.2 M Ammonium Nitrate