6QN8
Structure of bovine anti-RSV Fab B13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.908, 132.457, 118.183 |
| Unit cell angles | 90.00, 102.93, 90.00 |
Refinement procedure
| Resolution | 87.070 - 2.120 |
| R-factor | 0.23687 |
| Rwork | 0.235 |
| R-free | 0.26700 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.449 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.800 | 2.160 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.128 | |
| Number of reflections | 148202 | 7273 |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 99.2 | 97.6 |
| Redundancy | 6.8 | 6 |
| CC(1/2) | 0.990 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20.0% w/v Polyethylene glycol 3350 0.2 M Ammonium Nitrate |
