6QN4
Death-associated Protein Kinase 1 (DAPK1) catalytic and auto-regulatory domains with S289E and S308E mutations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.374, 77.478, 99.931 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.230 - 2.500 |
R-factor | 0.2173 |
Rwork | 0.215 |
R-free | 0.26610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b4l |
RMSD bond length | 0.009 |
RMSD bond angle | 0.947 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER (Phenix 1.9-1692) |
Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.931 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.131 | |
Rmeas | 0.161 | |
Rpim | 0.093 | |
Number of reflections | 26520 | 1440 |
<I/σ(I)> | 7.8 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.4 | 5.4 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | 16% PEG 4000, 0.1 M Tris pH 8.5, 0.2 M sodium acetate |