6QMN
Crystal structure of a Ribonuclease A-Onconase chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-30 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.442, 51.796, 66.093 |
| Unit cell angles | 90.00, 93.01, 90.00 |
Refinement procedure
| Resolution | 27.850 - 2.310 |
| R-factor | 0.2092 |
| Rwork | 0.205 |
| R-free | 0.29390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kf4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.639 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.380 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.126 | 0.368 |
| Number of reflections | 16885 | 1664 |
| <I/σ(I)> | 10.2 | 2.7 |
| Completeness [%] | 96.3 | 96.1 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.100 M Bis-Tris pH 6.5, 28 % W/V Polyethylene glycol monomethyl ether 2000 |
