6QM3
Crystal structure of a calcium- and sodium-bound mouse Olfactomedin-1 disulfide-linked dimer of the Olfactomedin domain and part of coiled coil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.705, 46.971, 104.952 |
| Unit cell angles | 90.00, 117.44, 90.00 |
Refinement procedure
| Resolution | 71.300 - 2.000 |
| R-factor | 0.180557826647 |
| Rwork | 0.179 |
| R-free | 0.21563 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5amo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.852 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.994 | 2.101 |
| High resolution limit [Å] | 1.890 | 1.891 |
| Rmerge | 0.060 | 0.751 |
| Rmeas | 0.072 | 0.893 |
| Rpim | 0.039 | 0.479 |
| Number of reflections | 29117 | 1457 |
| <I/σ(I)> | 10.3 | 1.7 |
| Completeness [%] | 92.2 | 77.2 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.998 | 0.551 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.08 M Mg Acetate, 30% PEG4000, 0.05 M Na Cacodylate pH 6.5 |
