6QLC

The ssDNA-binding RNA polymerase cofactor Drc from Pseudomonas phage LUZ7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000, 2.07333
Spacegroup name	I 41 2 2
Unit cell lengths	88.826, 88.826, 63.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.413 - 2.200
R-factor	0.2302
Rwork	0.227
R-free	0.28590
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.882
Data reduction software	XDS (Oct 2015)
Data scaling software	Aimless (0.5.25)
Phasing software	SHELX (2013/2)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.420	44.420	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.045	0.028	0.453
Rmeas	0.047	0.030	0.471
Rpim	0.013	0.010	0.126
Total number of observations	86244
Number of reflections	6690	119	571
<I/σ(I)>	31.3
Completeness [%]	100.0	99	100
Redundancy	12.9	10.5	13.7
CC(1/2)	0.999	0.999	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	1.8 M ammonium phosphate monobasic 0.1 M sodium acetate pH 4.6