6QL4

Crystal structure of nucleotide-free Mgm1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9794
Spacegroup name	P 41 2 2
Unit cell lengths	147.400, 147.400, 344.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.133 - 3.600
R-factor	0.244
Rwork	0.243
R-free	0.25230
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.976
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.133	49.133	3.690
High resolution limit [Å]	3.600	16.100	3.600
Rmerge		0.034	1.839
Rmeas		0.038	1.992
Number of reflections	44814	896	6118
<I/σ(I)>	7.96	36.74	1.07
Completeness [%]	99.8	94.7	98.4
Redundancy	6.9	5.999	6.761
CC(1/2)		0.999	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	8% PEG400, 3% isopropanol, 100 mM Na-citrate buffer (pH 5.5)