6QJF
Crystal Structure of the third PDZ domain of PSD-95 protein D332P mutant: space group C121, structure 1
Replaces: 5OI4Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.96998 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.900, 31.712, 71.651 |
Unit cell angles | 90.00, 99.51, 90.00 |
Refinement procedure
Resolution | 19.622 - 1.500 |
R-factor | 0.1546 |
Rwork | 0.152 |
R-free | 0.19890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k82 |
Data scaling software | Aimless (0.5.2) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.622 | 19.620 | 1.530 |
High resolution limit [Å] | 1.500 | 8.220 | 1.500 |
Rmerge | 0.051 | 0.022 | 0.626 |
Number of reflections | 53084 | 341 | 2577 |
<I/σ(I)> | 15.7 | ||
Completeness [%] | 97.6 | 91.4 | 96 |
Redundancy | 4.5 | 4.5 | 4.2 |
CC(1/2) | 0.999 | 0.999 | 0.818 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.16 M ammonium dihydrogenphosphate, 50 mM Tris, 20 % glycerol |