6QJA
Organizational principles of the NuMA-Dynein interaction interface and implications for mitotic spindle functions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-26 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.730, 112.850, 135.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.900 - 1.540 |
R-factor | 0.1717 |
Rwork | 0.170 |
R-free | 0.21470 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.670 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.910 | 1.570 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.036 | |
Rmeas | 0.039 | |
Rpim | 0.015 | |
Number of reflections | 104874 | |
<I/σ(I)> | 22.9 | 1.3 |
Completeness [%] | 100.0 | |
Redundancy | 6.5 | |
CC(1/2) | 1.000 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1 M Bis-Tris Propane, 0.2 M Magnesium Chloride, 28 % PEG 4000 |