6QJ4
Crystal structure of the C. thermophilum condensin Ycs4-Brn1 subcomplex bound to the Smc4 ATPase head in complex with the C-terminal domain of Brn1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.405, 82.378, 177.834 |
| Unit cell angles | 90.00, 98.77, 90.00 |
Refinement procedure
| Resolution | 47.743 - 5.800 |
| R-factor | 0.2926 |
| Rwork | 0.292 |
| R-free | 0.30150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6QJ2 and 6QJ3 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 175.756 | 47.743 | 5.800 |
| High resolution limit [Å] | 5.500 | 17.390 | 5.500 |
| Rmerge | 0.030 | 1.359 | |
| Rmeas | 0.145 | 0.037 | 1.608 |
| Rpim | 0.079 | 0.021 | 0.850 |
| Total number of observations | 25779 | ||
| Number of reflections | 7830 | 242 | 1147 |
| <I/σ(I)> | 8.1 | 22.2 | 0.5 |
| Completeness [%] | 97.6 | 88.9 | 99.6 |
| Redundancy | 3.3 | 2.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 280 | 0.1 M TRIS-HCl pH 8.5, 8 % PEG 8,000, 1 mM TCEP |
