6QIE
Crystal structure of DEAH-box ATPase Prp43-S387G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97620 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.640, 102.980, 118.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 77.848 - 2.700 |
R-factor | 0.2299 |
Rwork | 0.229 |
R-free | 0.25370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ltj |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 77.848 | 77.848 | 2.800 |
High resolution limit [Å] | 2.700 | 50.000 | 2.700 |
Rmerge | 0.112 | 0.011 | 1.478 |
Rmeas | 0.119 | 0.013 | 1.566 |
Number of reflections | 30459 | 4 | 3066 |
<I/σ(I)> | 14.78 | 64.61 | 1.64 |
Completeness [%] | 99.8 | 57.1 | 99.8 |
Redundancy | 9.115 | 2.75 | 9.244 |
CC(1/2) | 0.999 | 1.000 | 0.648 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM HEPES pH7, 3% PEG4000, 35% MPD |