6QIC

Crystal structure of DEAH-box ATPase Prp22-S837A with bound ssRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97620
Spacegroup name	P 21 21 21
Unit cell lengths	139.610, 139.960, 164.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.490 - 2.700
R-factor	0.2146
Rwork	0.212
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i3p
RMSD bond length	0.009
RMSD bond angle	1.511
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	106.490	106.490	2.820
High resolution limit [Å]	2.700	50.000	2.700
Rmerge	0.077	0.018	1.474
Rmeas	0.081	0.022	1.561
Number of reflections	88822	11	10719
<I/σ(I)>	19.56	48.14	1.69
Completeness [%]	99.7	55	99.8
Redundancy	9.251	2.818	9.442
CC(1/2)	1.000	1.000	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Bis-Tris propane pH 8.5, 250 mM NaF, 22% (w/v) PEG3350