6QH5
AP2 clathrin adaptor mu2T156-phosphorylated core in closed conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.920 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 121.037, 121.037, 257.671 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.520 - 2.560 |
R-factor | 0.22614 |
Rwork | 0.225 |
R-free | 0.25689 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vgl |
RMSD bond length | 0.015 |
RMSD bond angle | 1.793 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.520 | 2.620 |
High resolution limit [Å] | 2.560 | 2.560 |
Rmerge | 0.320 | |
Rmeas | 0.339 | |
Rpim | 0.109 | |
Number of reflections | 71252 | |
<I/σ(I)> | 8.4 | |
Completeness [%] | 99.8 | |
Redundancy | 17.9 | |
CC(1/2) | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289 | 20% PEG 1000; 100 mM Na+/K+ phosphate buffer (pH 7.2), 200 mM NaCl, and 10 mM DTT |