6QGI
Crystal structure of VP5 from Haloarchaeal pleomorphic virus 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-02-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9778 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.080, 93.220, 121.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.600 - 2.460 |
R-factor | 0.225 |
Rwork | 0.224 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HRPV6 VP5 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.990 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.600 | 2.520 |
High resolution limit [Å] | 2.460 | 2.460 |
Rmerge | 0.126 | 0.400 |
Rpim | 0.069 | |
Number of reflections | 16761 | 541 |
<I/σ(I)> | 10.2 | 2.2 |
Completeness [%] | 81.4 | 36.3 |
Redundancy | 4.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 12% (w/v) PEG 4000, 0.1 M Na-HEPES pH 7.5 and 0.1 M NaCl |