6QGI
Crystal structure of VP5 from Haloarchaeal pleomorphic virus 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-02-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.080, 93.220, 121.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.600 - 2.460 |
| R-factor | 0.225 |
| Rwork | 0.224 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HRPV6 VP5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.520 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.126 | 0.400 |
| Rpim | 0.069 | |
| Number of reflections | 16761 | 541 |
| <I/σ(I)> | 10.2 | 2.2 |
| Completeness [%] | 81.4 | 36.3 |
| Redundancy | 4.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 12% (w/v) PEG 4000, 0.1 M Na-HEPES pH 7.5 and 0.1 M NaCl |
