6QFL
Structure of the mitogen activated kinase kinase 7 active conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.000, 68.800, 85.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.058 - 2.200 |
R-factor | 0.2169 |
Rwork | 0.215 |
R-free | 0.24680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dyl |
RMSD bond length | 0.001 |
RMSD bond angle | 0.403 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.058 | 2.400 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.075 | 0.447 |
Number of reflections | 19157 | 3534 |
<I/σ(I)> | 17.11 | 3.54 |
Completeness [%] | 92.8 | 81.8 |
Redundancy | 5.8 | 5.93 |
CC(1/2) | 0.997 | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M Sodiumcitrate 20 % PEG3350 |