6QFC
Structure of an anti-Mcl1 scFv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.852, 40.458, 76.237 |
| Unit cell angles | 90.00, 110.55, 90.00 |
Refinement procedure
| Resolution | 38.730 - 1.960 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.21900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.003 | 1.833 |
| High resolution limit [Å] | 1.682 | 1.682 |
| Number of reflections | 28275 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 85.8 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12% PEG10k, 0.1M PCTP pH 7.4, 10% DMSO |
