6QEY
IMP1 KH1 and KH2 domains create a structural platform with unique RNA recognition and re-modelling properties
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.421, 32.987, 58.721 |
| Unit cell angles | 90.00, 103.14, 90.00 |
Refinement procedure
| Resolution | 37.210 - 2.200 |
| R-factor | 0.1845 |
| Rwork | 0.181 |
| R-free | 0.24550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.866 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.210 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmeas | 0.113 | 0.719 |
| Number of reflections | 8950 | 1423 |
| <I/σ(I)> | 7.28 | 1.89 |
| Completeness [%] | 97.0 | 96.8 |
| Redundancy | 2.74 | 2.73 |
| CC(1/2) | 0.993 | 0.607 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 51.4% PEG1000 150 mM MOPS pH 7.0 60 mM Sodium Iodide 4% acetonitrile |
