6QEY

IMP1 KH1 and KH2 domains create a structural platform with unique RNA recognition and re-modelling properties

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	41.421, 32.987, 58.721
Unit cell angles	90.00, 103.14, 90.00

Refinement procedure

Resolution	37.210 - 2.200
R-factor	0.1845
Rwork	0.181
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.866
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.210	2.260
High resolution limit [Å]	2.200	2.200
Rmeas	0.113	0.719
Number of reflections	8950	1423
<I/σ(I)>	7.28	1.89
Completeness [%]	97.0	96.8
Redundancy	2.74	2.73
CC(1/2)	0.993	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	51.4% PEG1000 150 mM MOPS pH 7.0 60 mM Sodium Iodide 4% acetonitrile