6QEV
EngBF DARPin Fusion 4b B6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 65 |
| Unit cell lengths | 194.770, 194.770, 123.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.690 - 2.700 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21800 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.690 | 2.797 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmeas | 0.338 | 3.030 |
| Number of reflections | 73165 | |
| <I/σ(I)> | 8.59 | |
| Completeness [%] | 99.8 | |
| Redundancy | 17.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 277.15 | MPD, sodium chloride, manganese chloride, PEG 20,000, MES |
