6QEP
EngBF DARPin Fusion 4b H14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-18 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.99987 |
Spacegroup name | P 65 |
Unit cell lengths | 192.690, 192.690, 123.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.280 - 2.600 |
R-factor | 0.179 |
Rwork | 0.177 |
R-free | 0.21500 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.280 | 2.693 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmeas | 0.172 | 3.263 |
Number of reflections | 80365 | |
<I/σ(I)> | 16.78 | |
Completeness [%] | 99.9 | |
Redundancy | 20.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.1 | 277.15 | MPD, PEG 20,000, sodium chloride, manganese chloride, MES |