6QEC

DNA binding domain of LUX ARRYTHMO in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	32.758, 51.795, 35.993
Unit cell angles	90.00, 110.55, 90.00

Refinement procedure

Resolution	30.673 - 1.900
R-factor	0.1942
Rwork	0.192
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lxu
RMSD bond length	0.008
RMSD bond angle	1.009
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.673	30.673	1.710
High resolution limit [Å]	1.660	7.430	1.660
Rmerge	0.043	0.021	0.555
Rmeas	0.053	0.026	0.734
Number of reflections	12519	143	790
<I/σ(I)>	9.01	27.55	1.2
Completeness [%]	93.5	87.7	79.2
Redundancy	2.55	2.622	1.935
CC(1/2)	0.998	0.999	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M BisTris Propane, pH 6.5, 20% PEG 3350 and 0.2 M sodium malonate