6QE7
anti-sigma factor domain-containing protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.340, 121.670, 65.940 |
| Unit cell angles | 90.00, 106.39, 90.00 |
Refinement procedure
| Resolution | 28.420 - 2.060 |
| R-factor | 0.19009 |
| Rwork | 0.187 |
| R-free | 0.25496 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | individual A and B chains |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.011 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.260 | 2.110 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Number of reflections | 60326 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 95.7 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 273 | 0.2M Am. Nitrate, 20% PEG 3350 |
