6QDM
Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97917 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.392, 97.610, 148.882 |
Unit cell angles | 90.00, 93.41, 90.00 |
Refinement procedure
Resolution | 48.805 - 3.800 |
R-factor | 0.2812 |
Rwork | 0.280 |
R-free | 0.30980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i2z |
RMSD bond length | 0.013 |
RMSD bond angle | 1.481 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 4.010 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmeas | 0.062 | 1.275 |
Number of reflections | 22138 | |
<I/σ(I)> | 9.1 | 1.1 |
Completeness [%] | 98.9 | 96.2 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 100 mM Tris HCl pH 8.5 32 % glycerol ethoxylate |