6QDI

anti-sigma factor domain-containing protein from Clostridium clariflavum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	104.600, 41.430, 73.310
Unit cell angles	90.00, 100.35, 90.00

Refinement procedure

Resolution	45.970 - 1.130
R-factor	0.14759
Rwork	0.146
R-free	0.17072
RMSD bond length	0.014
RMSD bond angle	1.662
Data reduction software	MOSFLM
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.440	1.160
High resolution limit [Å]	1.130	1.130
Number of reflections	111927
<I/σ(I)>	9.9
Completeness [%]	96.7
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M MES, pH 6.5, 12% PEG 20K