6QD6
Molecular scaffolds expand the nanobody toolkit for cryo-EM applications: crystal structure of Mb-cHopQ-Nb207
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976230 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.170, 92.920, 244.220 |
| Unit cell angles | 92.05, 96.93, 112.15 |
Refinement procedure
| Resolution | 41.450 - 2.840 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.23900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.450 | 2.900 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.039 | 0.469 |
| Rmeas | 0.055 | 0.663 |
| Number of reflections | 129248 | 7733 |
| <I/σ(I)> | 11.65 | 1.45 |
| Completeness [%] | 95.6 | 94.5 |
| Redundancy | 1.78 | 1.76 |
| CC(1/2) | 0.999 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.2 M ammonium citrate, 17 % PEG3350, 10 % Glycerol |
