6QCY
MloK1 model from single particle analysis of 2D crystals, class 2 (intermediate conformation)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.7 |
| Number of Particle | 231688 |
| Software | PHENIX,FOCUS,FREALIGN,CTFFIND,CTFTILT,iMODFIT |
| Symmetry | C4 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
