6QCX
Crystal structure of influenza B polymerase initiation state with capped 15-mer RNA primer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.966 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 200.407, 200.407, 256.372 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 173.560 - 3.080 |
R-factor | 0.20358 |
Rwork | 0.202 |
R-free | 0.22582 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5msg |
RMSD bond length | 0.002 |
RMSD bond angle | 1.141 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.270 |
High resolution limit [Å] | 3.080 | 3.080 |
Rmeas | 0.086 | 0.888 |
Number of reflections | 83143 | |
<I/σ(I)> | 14.3 | 1.7 |
Completeness [%] | 75.2 | 61 |
Redundancy | 4.8 | |
CC(1/2) | 0.999 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 277 | Influenza B polymerase at 9 mg per ml in was mixed with 40 microM vRNA 5 prime end 14-mer, 40 microM vRNA 3 prime end 21-mer and 80 microM 15-mer capped RNA. The mother liquor contained 200 mM di-ammonium phosphate and 100 mM sodium acetate between pH 4.0 and 4.4. |