6QCW
Crystal structure of influenza B polymerase initiation state with capped 14-mer RNA primer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.977 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 200.354, 200.354, 256.466 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.880 |
R-factor | 0.21548 |
Rwork | 0.215 |
R-free | 0.23391 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5msg |
RMSD bond length | 0.003 |
RMSD bond angle | 0.731 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.120 |
High resolution limit [Å] | 2.880 | 2.880 |
Rmeas | 0.185 | 1.500 |
Number of reflections | 81417 | |
<I/σ(I)> | 8.2 | 1.8 |
Completeness [%] | 60.7 | 60.9 |
Redundancy | 8.2 | 10 |
CC(1/2) | 0.995 | 0.677 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 277 | Influenza B polymerase at 9 mg per ml in was mixed with 40 microM vRNA 5 prime end 14-mer, 40 microM vRNA 3 prime end 21-mer and 80 microM 14-mer capped RNA. The mother liquor contained 200 mM di-ammonium phosphate and 100 mM sodium acetate between pH 4.0 and 4.4. |