6QCU

Crystal structure of a Fab portion of the anti EBOV 3T0331 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-05
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.87313
Spacegroup name	P 65 2 2
Unit cell lengths	91.095, 91.095, 215.434
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	78.891 - 1.560
R-factor	0.1771
Rwork	0.176
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.144
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_3297: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.900	1.616
High resolution limit [Å]	1.560	1.560
Rmerge	0.132
Rmeas	0.138
Rpim	0.038	0.254
Number of reflections	75996	7436
<I/σ(I)>	10.3
Completeness [%]	100.0	100
Redundancy	13	13.2
CC(1/2)	0.996	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15M LiSo4 monohydrate, 0.1M Citric Acid pH3.5, 18% PEG 6000, 5% Ethylen Glycol