6QC0
PCNA complex with Cdt2 C-terminal PIP-box peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.872600 |
Spacegroup name | P 61 |
Unit cell lengths | 151.369, 151.369, 91.489 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.730 - 3.500 |
R-factor | 0.19836 |
Rwork | 0.196 |
R-free | 0.25164 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.806 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.700 | 3.830 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 14850 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 97.1 | |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 10% (w/v) PEG 6000 100 mM MES/HCl |