6QBW
Structure of the HTLV-2 integrase catalytic core domain in complex with calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 291 |
Detector technology | PIXEL |
Collection date | 2018-05-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 112.082, 112.082, 112.082 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.120 - 2.400 |
Rwork | 0.247 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qbt |
Data reduction software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.120 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.028 | 0.310 |
Number of reflections | 9932 | 1872 |
<I/σ(I)> | 13.8 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 1.8 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 100 mM Tris pH 8, 18% PEG 6000, 250 calcium chloride dihydrate |