6QBN
structure of the core domaine of Knr4, an intrinsically disordered protein from Saccharomyces cerevisiae - mutant S200D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976256 |
| Spacegroup name | P 62 |
| Unit cell lengths | 103.110, 103.110, 93.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.180 - 2.400 |
| R-factor | 0.18175 |
| Rwork | 0.180 |
| R-free | 0.21691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j1b |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.660 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.300 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 1.345 | |
| Number of reflections | 20213 | 1121 |
| <I/σ(I)> | 0.046 | 1.3 |
| Completeness [%] | 95.3 | 68.9 |
| Redundancy | 8 | 5.8 |
| CC(1/2) | 1.000 | 0.460 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | PEG 3000 - 6000 15-24 % (w/v) Bicine buffer 0.1 M |
