6QB7

Structure of the H1 domain of human KCTD16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 21 21 21
Unit cell lengths	74.427, 105.620, 107.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 2.230
R-factor	0.232
Rwork	0.230
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Unpublished
RMSD bond length	0.002
RMSD bond angle	0.431
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.310
High resolution limit [Å]	2.230	2.230
Number of reflections	42017
<I/σ(I)>	9.22
Completeness [%]	97.4
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Mother liquor: 0.8M ammonium phosphate monobasic -- 0.8M ammonium phosphate dibasic 1:1 drops with protein in: 300 mM NaCl, 50 mM HEPES pH 7.5, 1 mM TCEP, 5 mM DTT