6Q8W
Respiratory complex I from Thermus thermophilus with bound Aureothin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.980, 340.500, 264.270 |
| Unit cell angles | 90.00, 100.44, 90.00 |
Refinement procedure
| Resolution | 49.710 - 3.400 |
| R-factor | 0.2173 |
| Rwork | 0.213 |
| R-free | 0.23420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hea |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.988 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3026: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.710 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.230 | 1.356 |
| Rpim | 0.079 | 0.775 |
| Number of reflections | 211093 | 21974 |
| <I/σ(I)> | 10.9 | 1 |
| Completeness [%] | 92.7 | 66.3 |
| Redundancy | 8.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 296 | 100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside |
