6Q8V

Structure of the standard kink turn HmKt-7 variant A2bm6A.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9197
Spacegroup name	P 63 2 2
Unit cell lengths	70.660, 70.660, 48.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.330 - 2.090
R-factor	0.2057
Rwork	0.205
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cs1
RMSD bond length	0.005
RMSD bond angle	1.004
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.330	2.130
High resolution limit [Å]	2.090	2.090
Rmerge	0.036	2.280
Rpim	0.011	0.670
Number of reflections	4521	218
<I/σ(I)>	29.7
Completeness [%]	99.0	100
Redundancy	12.1	12.2
CC(1/2)	1.000	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	0.1 M Potassium chloride, 0.002 M Spermine tetrahydrochloride, 0.05 M Bis-Tris 7.0, 15 % w/v PEG 2000 MME